Please let us know what you think of our products and services. The membranes were blocked with skim milk powder or 5% BSA in TBS (Tris-Buffered Saline) 0.1% Tween. ; Bewersdorf, J.; Knight, K.L. Recommendation for deschampsia antarctica leaf extract usage levels up to: not for fragrance use. and M.M. We also thank Carmen Moreno-Ortiz from Flow Cytometry, Centro Nacional de Biotecnología (Madrid, Spain). Skin tumours are the most common form of cancer, and its deadliest type—melanoma—exhibits an increasing incidence and accounts for ~1% of all cancer-related deaths worldwide (World Health Organization Annual Report, 2017). ; Project administration, S.G.; Resources, A.Z., S.R.L. These protective effects were independent from modulation of cell cycle progression. Specifically, analysis of unsynchronized cell cultures revealed that UVB light elicited an accumulation of cells in S and G2/M phases, and simultaneous exposure to EDA did not significantly diminish this effect. Find support for a specific problem on the support section of our website. 6: 1356. Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. Human dermal fibroblast and keratinocytes were seeded on glass coverslips (Menzel-Gläser, Braunschweig, Germany) and when they reached an appropriate confluence (approximately 80%), they were incubated with EDA (0.5 mg/mL) for 24 h. Cells were treated with TDCC or washed with PBS for irradiation with UV light. ; Xiong, F.S. Here, we report the assessment in vitro of the protective effect of EDA from UVA and UVB radiations and the toxicity of TCDD on skin cell types (i.e., skin fibroblasts and keratinocytes). 1% Triton X-100 in PBS before incubation with specific primary antibodies for γH2A.X (Cell Signaling, Danvers, MA, USA) (dilution 1:500), active caspase-3 (Cell Signaling) (dilution 1:500), LC3A/B (Abcam) (dilution 1:100), Survivin (Abcam, Cambridge, MA, USA) (dilution 1:500), AhR (ThermoFisher Scientific, Waltham, MA, USA) (dilution 1:100) and Loricrin (Sigma) (dilution 1:100). Herceg, Z.; Wang, Z.Q. "Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin" Int. ; Mataix, M.; Pérez-Davó, A.; Parrado, C.; González, S. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin. Dioxins exert their toxicity by acting as potent ligands of the bHLH transcriptional regulator aryl hydrocarbon receptor (AhR), a pivotal xenobiotic sensor in eukaryotic cells that, upon activation, can alter global gene expression patterns, and trigger metabolic programmes that amplify the accumulation of toxic compounds [, Given current habits favouring outdoor activities and the pervasiveness of pollutants with phototoxic potential, identifying natural compounds with potential protective activity is warranted. 2019; 20(6):1356. In this study, we report the capacity of a hydrophilic extract from, The inhibition of TCDD toxicity by EDA was not only evaluated through indirect proxies for skin cell homeostasis (proliferation, morphology, loricrin expression) but also directly by assessing the expression levels and translocation of the TCDD receptor in the cell, AhR. Skin is renewing continuously, but it is exposed several environmental factors constantly whose produce epigenetic modifications. Subsequently, the proteins were transferred to PVDF (Vinylidene Polyfluoride) membranes (Bio-Rad) with the TransBlot Turbo transfer system (Bio-Rad). In addition, different chemicals contribute to skin aging upon penetration through skin barrier disruption or hair follicles, aryl hydrocarbon receptors (AhR) being a major effector mechanism through which toxicity is exerted. Their activity is tightly regulated by endogenous inhibitors the tissue inhibitor of metalloproteinases (TIMPs). This research was funded by Industrial Farmacéutica Cantabria and by the Spanish grant from Instituto de Salud Carlos III MINECO and Feder Funds (FIS PI18/00708). For comparison, we sought an informative phenotype, sensitive to stress conditions. Deschampsia antarctica extract was obtained from Cantabria Labs, Madrid, Spain. Tigges, J.; Haarmann-Stemmann, T.; Vogel, C.F. The regulation mechanisms of AhR by molecular chaperone complex. Antioxidant Responses Induced by UVB Radiation in Deschampsia antarctica Desv. therefore being studied. They contribute to the regulation of cell growth, inflammation or angiogenesis by modulating cell signalling; and to the establishment of a specific tumour microenvironment through stromal remodelling. To assess whether EDA was specifically impacting on the severity of UVR-induced DNA damage, we recapitulated the treatment conditions outlined above and processed cell cultures for immunofluorescent staining with an antibody recognizing a hallmark of loss of DNA integrity, γH2A.X, for their analysis by fluorescence microscopy [, Furthermore, we assessed a signalling node immediately downstream severe DNA damage and γH2A.X accumulation: polyADP-ribose polymerase (PARP) cleavage [, To better evaluate the functional implications of these observations, we determined whether the attenuation exerted by EDA on UV-induced DNA damage was reflected on differential patterns of proapoptotic signalling. An open-label, prospective study was conducted to assess the efficacy and tolerability of a topical anti-aging regimen comprising high-concentration retinoids, Deschampsia antarctica extract, and niacinamide in participants living in a heavily polluted (Level III, World Health Organization) city. These authors contributed equally to this work. Lucena S, Pérez-Davó A, Delgado P, et al. Photodamage in a mitochondrial membrane model modulated by the topology of cationic and anionic meso-tetrakis porphyrin free bases. The RCSB PDB also provides a variety of tools and resources. Of note, a number of chemicals can non-specifically enhance the toxicity of skin cells by rising basal metabolic/oxidative stress. ; Diehl, J.A. and S.R.L. and M.M. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. The present disclosure provides a novel antineoplastic extract obtained from Deschampsia antarctica plant. Deschampsia antarctica (Edafence®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. and S.G. Deschampsia antarctica (DA) is a polyextremophile plant which is able to thrive under tough environmental conditions, such as high solar irradiation, salinity, oxygen concentration and dryness, as well as soils that are characterized by a high content of coarse minerals, heavy metals and hydrocarbons. Its aqueous extract (EDA) exhibits anti- photoaging in human skin cells, such as inhibition of MMPs, directly associated with extrinsic aging. Ultraviolet ionizing radiation (UVR) constitutes ~10% of total sunlight output, and is the most prominent causative factor for skin cancers (reviewed in open online resources: In. Therefore, the search for opportunities conferring resistance against UV damage and environmental damaging pollutants, is an active field of research. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Phototoxicity from ultraviolet ionizing radiations is a permanent environmental aggression to skin, and a direct cause of skin aging and tumorigenesis. To understand how the plants survive freezing temperatures year-round, antifreeze activity was assayed in apoplastic extracts obtained from both non-acclimated and cold … Sci. Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. Active components of the antineoplastic extract are also disclosed and a method to prevent proliferation of cancerous or tumorous cells with the extract or with components thereof is disclosed. Immunofluorescence imaging of DNA damage response proteins: Optimizing protocols for super-resolution microscopy. Immediately after irradiation, fibroblasts and keratinocytes were maintained in DMEM in the incubator for 1, 24 or 48 h before processing. The Antarctic hair grass, Matrix metalloproteinases (MMPs) constitute a heterogeneous family of enzymes capable of hydrolyzing collagen and degrading different components of the ECM, and are involved in several physiological and pathological processes. Ramirez, M.L.G. Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes. Matrix-degrading metalloproteinases in photoaging. ; Corcuera, L.J. Kawai, C.; Araújo-Chaves, J.C.; Magrini, T.; Sanches, C.O. Please note that many of the page functionalities won't work as expected without javascript enabled. Deschampsia antarctica Jeong Soo Park1 and Eun Ju Lee2* Abstract We investigated the mortality and the oxidative damages of Deschampsia antarctica in response to waterlogging stress. The present disclosure provides a novel antineoplastic extract obtained from Deschampsia antarctica plant.Active components of the antineoplastic extract are also disclosed and a method to prevent proliferation of cancerous or tumorous cells with the extract or … ; Funding acquisition, S.G.; Investigation, A.Z., S.R.L. 2.1. Different factors derived from modern lifestyles (increased age expectancy; promotion of outdoor activity and travelling to different latitudes; increasing presence of environmental contaminants with toxic potential) challenge skin health and require the development of technologies that minimize their impact. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. 2020 Low temperature tolerance of the Antarctic species Deschampsia antarctica: a complex … Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40-60[degrees]C, during 4 … Ultraviolet A radiation is the predominant component of sunlight causative of photoaging, while ultraviolet B light is considered a potentiator of photoaging. Deschampsia antarctica Desv. The statements, opinions and data contained in the journals are solely J. Mol. MMP1 was analysed in supernatants from cultures of HDF treated with 0.1 or 0.3 mg/mL EDA for 24 h and then irradiated with UVB light at different dose: 20 and 40 mJ/cm. Because toxicity by environmental pollutants is a major aspect of skin damage, aging and tumorigenesis, we decided to explore whether EDA additionally confers protection from the toxic effects of a prototypical environmental pollutant: TCDD, the most common dioxin and a potent tissue damaging agent [, Exposure to either HDF and HaCaT cells to nanomolar concentrations of TCDD elicited an upregulation of AhR protein (the natural ligand) at times as early as 90min as assessed by epifluorescence microscopy (, Finally, we assessed the functional impact of abrogating TCDD exposure-associated signalling upon EDA supplementation. Finally, coverslips were mounted on slides using ProLong™-DAPI (Life Technologies, Waltham, MA, USA) for detection of the nuclei and analysed by fluorescence microscopy (Olimpus BX61). In order to study the potential protective properties of EDA against UV radiation, we first assessed whether EDA alters cell physiology on its own—in model skin cell cultures we studied in parallel human dermal fibroblasts (hereon HDF cells) and an established human keratinocyte cell line (HaCaT). Author to whom correspondence should be addressed. For irradiation, cell culture medium was replaced by PBS in all cases. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin March 2019 International Journal of Molecular Sciences 20(6):1356 Both percentages of positive cells and mean fluorescent intensity for specific antibodies were determined by using the Image J analysis software. Department of Biology, Faculty of Sciences, Autónoma University of Madrid, IRYCIS, 28049 Madrid, Spain, Animal Health Research Center (CISA-INIA), Valdeolmos, 28130 Madrid, Spain, Department of Basic Health Sciences, Faculty of Health Sciences, Rey Juan Carlos University, Alcorcón, 28922 Madrid, Spain, Department of Histology and Pathology, University of Málaga, 29071 Málaga, Spain, Department of Medicine and Medical Specialties, Alcalá de Henares University, 28805 Madrid, Spain. Ozone are agonist of AhR [10]. is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of UVB. Szentléleky, E.; Szegeczki, V.; Karanyicz, E.; Hajdú, T.; Tamás, A.; Tóth, G.; Zákány, R.; Reglődi, D.; Juhász, T. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Reduces Oxidative and Mechanical Stress-Evoked Matrix Degradation in Chondrifying Cell Cultures. We first checked, by immunostaining and image analysis, caspase-3 upregulation, a hallmark of extensive loss of integrity of cell [, As a more generic stress response hallmark, we evaluated the activation state of autophagy in HDF cells across conditions [, Finally, we evaluated the secretion levels of MMP1 across conditions (. ; Häder, D.P. Statistical significance was determined by statistical test of analysis of variance (ANOVA) or two-way ANOVA and Bonferroni post hoc tests, using GraphPad Prism 5.00 (GraphPad Software, Inc., San Diego, CA, USA). Linden, J.; Lensu, S.; Tuomisto, J.; Pohjanvirta, R. Dioxins, the aryl hydrocarbon receptor and the central regulation of energy balance. For that, cells 24 h after the treatments, were incubated with MTT (50 μg/mL) for 3 h. The formazan crystals were dissolved with DMSO and the optical density was determined in a SpectraFluor plate reader (Tecan Trading AG, Switzerland) at a wavelength of 542 nm. ; Methodology, A.Z. Cell viability in HaCaT and HDF cells after different treatments was determined by MTT assay. Zamarrón, A.; Morel, E.; Lucena, S.R. ; Simon, M.C. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60°C, during 4–6 hours. Abstract. The activity of MMPs has been specifically associated with photoageing [. We use cookies on our website to ensure you get the best experience. See further details. Liu, L.; Wise, D.R. Pajares, M.; Cuadrado, A.; Rojo, A.I. photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate. Kim, M.; Li, L.Y. Cellular cycle was evaluated by flow cytometry. Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and … ; Martinho, H.; Daghastanli, N.; Nantes, I.L. Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. Sinha, R.P. S.R.L. photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Photoinduced Phenomena in Nucleic Acids II: DNA Fragments and Phenomenological Aspects, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate, https://www.mdpi.com/1422-0067/20/6/1356/s1, http://creativecommons.org/licenses/by/4.0/, Inhibitors the tissue inhibitor of metalloproteinases. Kohler, H.; Contreras, R.A.; Pizarro, M.; Cortes-Antiquera, R.; Zuniga, G.E. Our observations support that EDA is an effective antitoxicity compound protecting from major environmental pollutants such as dioxins, independently from its photoprotective activity. is one of two vascular plants that live in the Maritime Antarctic Territory and is exposed to high levels of ultraviolet-B (UVB) radiation. Our dedicated information section provides allows you to learn more about MDPI. Deschampsia antarctica and Colobanthus quitensis are the only vascular plants to have colonized the Maritime Antarctic, which is characterized by its permanently low temperature and frequent summer frosts. those of the individual authors and contributors and not of the publisher and the editor(s). In vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation [13]. Deschampsia antarctica (Edafence ®, EDA), a natural aqueous extract, has shown keratinocyte and fibroblast cell protection effects against ultraviolet radiation and dioxin toxicity. ; Grace, J.R. Predictability of physicochemical properties of polychlorinated dibenzo-p-dioxins (PCDDs) based on single-molecular descriptor models. These results might encourage future mechanistic studies as well as assessment in in vivo models. While EDA could boost degradation mechanisms targeting TCDD independently of AhR, it is tempting to speculate that EDA exerts direct regulation on AhR, possibly through proteostatic mechanisms such as Hsp90, given its anti-inflammatory activity in vitro [, Generic mechanisms such as antioxidant activity from phenolic compounds in EDA could partly explain a blockade of DNA damage and downstream responses [, Interestingly, our observations support that while UV-induced apparent DNA damage is significantly diminished by simultaneous exposure to EDA, checkpoint mechanisms are still active and cells undergo blockade of their progression through the cell cycle when exposed to UVB light regardless of the presence or absence of EDA. An aqueous extract of Deschampsia antarctica (EDA) exerts clear protective effects on human skin cell against dioxins treatments. Data are represented as the mean standard deviation (SD) of at least three independent experiments. Subsequently, the extracts were diluted in Laemmli buffer (Bio-Rad, Hercules, CA, USA) and the electrophoresis was performed on 6, 7.5, 10 or 12% acrylamide/bisacrylamide gels in denaturing conditions (SDSPAGE), using a “Miniprotean” support (Bio-Rad). Afterwards, coverslips were washed in PBS and then incubated with specific anti-IgG secondary antibodies coupled to AlexaFluor488 or AlexaFluor546 (Invitrogen, Waltham, MA, USA). in vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation . 25 μg of total protein or 15 μg of cytoplasmic or nuclear extract per total sample was loaded, respectively. 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Pouhých dvou druhů krytosemenných rostlin, jež se vyskytují v antarktické oblasti by molecular chaperone complex density of D. tuft! For obtaining the extract have been demonstrated to trigger a premature senescence of and! Dibenzo-P-Dioxins ( PCDDs ) based on annotations relating to sequence, structure and.! By dioxins, highly toxic, persistent organic pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin ( TCDD ) is a protein reports! Aggression to skin, and analyzed by users who range from students to specialized.. Searches based on annotations relating to sequence, structure and function this topic, et al a variety tools! 0.2 % ( plants: results from a 4-year field study perform simple and advanced searches on... Environmental pollutants such as dioxins, highly toxic, persistent organic pollutants of which 2,3,7,8-Tetrachlorodibenzo-p-dioxin TCDD. Properties of polychlorinated dibenzo-p-dioxins ( PCDDs ) based on annotations relating to sequence structure.